Overview

mRNA libraries are prepared starting with total RNA.Library quality is checked with bioanalyzer. Concentration is measured with picogreen.

Technology

Illumina TruSeq mRNA library preparation kit and Kapa mRNA hyper kit are used for the library preparation.

Key Features

We prefer that customers provide 100-2000ng total RNA with RIN equal or above 7.0. But for the customers who have limited quantity and quality total RNA, we ask as low as 50ng. The RNA sample volume is up to 50μl in nuclease free H₂O.