genomics block

mRNAseq-Strand Specific Library Preparation

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Overview

mRNA libraries are prepared starting with total RNA.Library quality is checked with bioanalyzer. Concentration is measured with picogreen.

Technology

Illumina TruSeq mRNA library preparation kit and Kapa mRNA hyper kit are used for the library preparation.

Key Features

We prefer that customers provide 100-2000ng total RNA with RIN equal or above 7.0. But for the customers who have limited quantity and quality total RNA, we ask as low as 50ng. The RNA sample volume is up to 50μl in nuclease free H2O.

genomics block

DNA/RNA Extraction

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Overview

DNA/RNA extraction service for different types of cells, tissues including FFPE samples

Technology

Different type of kits from Qiagen are used for different type of samples

Key Features

The quality check with bioanalyzer and Nanodrop is included in the extraction service

genomics block

Sample Quality Control

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Overview

Check the RNA/DNA quality to get RIN/DIN and concentration

Technology

Agilent 2100 Bioanalyzer system and Agilent 4150 TapeStation Systems are used to get the RIN/DIN, Nanodrop and Picogreen to measure the concentration

Key Features

RIN/DIN > 7 is considered good quality

Carlos Arana

Carlos Arana

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Chaoying Liang

Chaoying Liang

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Jinchun Zhou

Jinchun Zhou

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IRF1 governs the differential interferon-stimulated gene responses in human monocytes and macrophages by regulating chromatin accessibility

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Deep sequencing reveals a DAP1 regulatory haplotype that potentiates autoimmunity in systemic lupus erythematosus

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RAD51 interconnects between DNA replication, DNA repair and immunity

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