Overview

Simoa (Single Molecular Array) HD-X analyzer at the IPC is one of the most sensitive automated bead-based immunoassay platforms with multiplexing and custom assay properties.

Technology

• Paramagnetic beads coupled to capture antibodies are incubated with the sample analyte of interest and detection antibodies are added.
• The beads are coupled to an immunocomplex consisting of the capture antibody-analyte-detection antibody.
• The individual beads are trapped in femtoliter-sized wells along with a fluorogenic enzyme substrate, those that are bound by the immunocomplex are detected by fluorescence that is converted to a digital read.

Key Features

• High throughput and automated
• 1000x more sensitive compared to traditional immunoassays
• Multiplexing up to six biomarkers to as low as fg/ml concentration range

Availability

Panels are available for the following markers:

• Angiogenesis and Vascular panels
• Bone metabolism panels
• Cytokine and chemokine panels
• Inflammation panels
• Intracellular signaling panels
• Neurodegeneration panels
• Toxicology panels (cardiac, kidney, liver, muscle injuries and natriuretic peptide)
• SARS-CoV-2 panels