Native RNA Sequencing on Nanopore
Overview
This assay allows complete quantification and characterization of native RNA or cDNA molecules present in a sample. A sequencing library is constructed on poly-A enriched RNA followed by sequencing on MinION/Mk1c platform from Oxford Nanopore.
Technology
We use the following commercially available kits and platforms for sequencing library preparation and sequencing.
- Nanopore Native RNAseq library prep
- MinION R9.4.1 sequencing
- EPI2ME analysis pipeline
Key Features
- Full-length transcripts
- Unambiguous identification of splice variants and fusion events
- Accurate isoform quantification
- No PCR bias
- Detects base medications (epigenetic changes) alongside nucleotide sequence