microbiome block

Native RNA Sequencing on Nanopore

Overview

This assay allows complete quantification and characterization of native RNA or cDNA molecules present in a sample. A sequencing library is constructed on poly-A enriched RNA followed by sequencing on MinION/Mk1c platform from Oxford Nanopore.

Technology

We use the following commercially available kits and platforms for sequencing library preparation and sequencing.

  • Nanopore Native RNAseq library prep
  • MinION R9.4.1 sequencing
  • EPI2ME analysis pipeline

Key Features

  • Full-length transcripts
  • Unambiguous identification of splice variants and fusion events
  • Accurate isoform quantification
  • No PCR bias
  • Detects base medications (epigenetic changes) alongside nucleotide sequence