Overview

The Agilent 2100 Bioanalyzer system is an established automated electrophoresis solution for the sample quality control of biomolecules. The system integrates an instrument, data processing software, reagents, and a microfluidic chip specific for DNA, RNA, or protein analysis.

Technology

Conventional gel electrophoretic assays have been transferred to a chip format. DNA and protein samples are quantitated with the help of an upper marker or known standard while RNA samples are run alongside a ladder and quantitated. For total RNA assays, ribosomal ratio is determined which is an indicator of the integrity of the sample.

Key Features

• The Agilent RNA 6000 Pico kit will accommodate up to 11 samples with concentration ranging from 50-5000 picograms per microliter for samples in water.
• The Agilent Small RNA kit will accommodate up to 11 samples and will separate RNA fragments in the range of 6 to 150 nucleotides with a total RNA concentration 1-100 nanograms per microliter.”
• The Agilent RNA 6000 Nano kit will take a maximum of 12 samples with the concentration range in 25 to 500 nanograms per microliter.
• The Agilent DNA 1000 kit will accommodate up to 12 samples in the size range of 25 to 1000 bp and a concentration range of 0.1 to 50 nanograms per microliter.

Bioanalyzer Service

The service fee is $5 per sample. Please submit RNA samples in the concentration range of 15 to 500 ng/μl for use with the Nano chip. Bioanalyzer only orders with RNA samples below 15 ng/μl may use the Pico chip. An RNA Integrity Number (RIN) should be greater than 8.0.