microbiome block

Full-length 16S rRNA Gene Sequencing

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Overview

This assay sequences full-length 16S rRNA gene sequence (V1-V9) which allows high resolution taxonomic classification of microbes in a given specimen. We provide this service using Oxford Nanopore long-read sequencing technology. Full length 16S rRNA gene is amplified and sequenced on MinION /Mk1c R9.4.1. Flow cell. We input about 50-100 nanogram DNA for this assay.

Technology

We use the following commercially available kits and platforms for sequencing library preparation and sequencing.

  • Oxford Nanopore Library prep kit
  • MinION R9.4.1 flow cell
  • EPI2ME data analysis pipeline

Key Features

Rapid and high resolution taxonomy of bacteria.