Overview

This assay sequences targeted hypervariable regions i.e. v3-v4 of Bacterial 16S rRNA gene for identification and taxonomic classification of bacterial communities in a given specimen. We provide services to sequence various hypervariable regions (V1-V9) of 16S rRNA gene for bacterial taxonomy and phylogeny. Customers can select the hypervariable region/s based on their sample source and study aims. We input about 10-100 nanogram DNA for this assay.

Technology

We use the following commercially available kits and platforms for sequencing library preparation and sequencing.

• Zymo Research Library prep Kits
• MiSeqDx 500 cycle sequencing
• MiSeqDx 600 cycle sequencing

Key Features

OTU clustering analysis for microbiome profiling